http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110894483-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de020b7dcbcaf745ca743fa95fa94853 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-22 |
| filingDate | 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb5003941456eb4c2ba437612a23c4f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7edd3975d8de91a7b77174938c820935 |
| publicationDate | 2020-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110894483-A |
| titleOfInvention | A screening method for bacteria degrading atrazine and cellulose |
| abstract | The invention relates to the technical field of microorganism separation, in particular to a screening method for bacteria degrading atrazine and cellulose. A screening method for bacteria degrading atrazine and cellulose, comprising the steps of: taking forest soil with plants, straws and fallen leaves falling all year round, inoculating it on an enrichment medium by a concentration gradient method, and culturing until a single colony appears, Transfer to carboxymethyl cellulose as the sole carbon source plate for purification until a single colony appears; (2) serially passage the single colony on the carboxymethyl cellulose as the sole carbon source plate; (3) transfer the single colony to a Microcrystalline cellulose powder is the only carbon source on the plate, until a single colony appears, and it is continuously passaged; (4) the single colony is transferred to the atrazine directional screening medium by the plate streaking method, and cultivated until the single colony appears; ( 5) Amplify the 16SrDNA of a single colony, compare and identify the 16SrDNA, and the comparison is the retention of bacterial genus. |
| priorityDate | 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.