http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110892066-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e0bf0928b608797b70bbc2b91c7e52e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2018-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61ce9b8e3035f7e1131dd28ba8f8851d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d0d98bd92d579f16ed5ca21b5bab177 |
| publicationDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110892066-A |
| titleOfInvention | Methods of transforming bacterial cells |
| abstract | The present invention relates to the production of a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA, resulting in DNA containing 5-methylcytosine in the recognition sequence GCNGC, and wherein the DNA The methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO:33. In addition, the present invention relates to the use of such a DNA methyltransferase for producing bacterial transformants, said use comprising the steps of: (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide encoding the A polynucleotide sequence of a DNA methyltransferase to produce methylated DNA, and (b) to transfer the methylated DNA from a first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell contains a protein capable of degrading A restriction endonuclease that does not degrade the DNA but cannot degrade the methylated DNA. |
| priorityDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1384.