| abstract |
The invention discloses an expression vector that can controllably up-regulate ACE2 for targeted prevention and treatment of hypoxic pulmonary hypertension. The invention constructs an HRE-enhanced and Tie2 promoter-driven ACE2 expression vector, which is specifically located in vascular endothelial cells through the Tie2 promoter, and at the same time, uses HIF-1α to express and specifically activate HRE only in hypoxic pulmonary vascular endothelial cells, thereby up-regulation ACE2 expression. It is found in the present invention that the constructed expression vector not only has targeting, but also can controllably and effectively upregulate the expression of ACE2 in hypoxic pulmonary microvascular endothelial cells according to the degree of hypoxia, thereby regulating pulmonary artery smooth muscle cells and realizing controllable hypoxia It can inhibit the contraction of pulmonary artery and reverse the structural reconstruction of pulmonary artery in a targeted and targeted manner, and provide effective means and strategies for the prevention and treatment of hypoxic pulmonary hypertension and other diseases. |