| abstract |
The invention discloses a recombinant adeno-associated virus (rAAV) vector carrying human sperm protein 17 (Sperm protein 17, SP17) antigen gene, and proves its application value. The rAAV vector is obtained by inserting the SP17 antigen gene into the AAV starting vector constructed by the inventors. The research of the present invention proves that the rAAV viral vector can effectively transport the SP17 antigen gene carried by it into the human monocyte-dendritic cell line, which can be used to stimulate the effector cells of cellular immunity, that is, SP17 antigen-specific cytotoxicity T lymphocyte (CTL) production. Experiments show that CTLs induced by DCs infected with the recombinant adeno-associated virus vector carrying the SP17 antigen gene of the present invention can effectively lyse SP17 antigen-positive malignant tumor cells. Therefore, the recombinant adeno-associated virus vector carrying the SP17 antigen gene or its related products of the present invention has practical value, and can be used to prepare SP17 antigen-specific CTL, kill SP17 antigen-positive malignant tumor cells, and be used for anti-SP17 antigen-positive cells. Targeted T-cell immunotherapy of malignant tumors. |