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filingDate 2019-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110878340-A
titleOfInvention Fluorescence quantitative PCR detection method for authenticity identification and doping analysis of potato starch
abstract The invention discloses a fluorescent quantitative PCR detection method for potato starch authenticity identification and doping analysis, comprising the following steps: A. extracting its DNA from starch to be tested; B. designing fluorescent quantitative PCR primers; C. Fluorescence quantitative PCR reaction system and operating procedures; D. Identification and analysis: If there is an amplification curve in the obtained result and two specific conditions are met at the same time, the tested product is identified as true potato starch; if the obtained result shows If there are multiple amplification curves, and one of the curves satisfies two specific conditions at the same time, the tested product is identified as adulterated potato starch; if the obtained result shows multiple curves, but all the curves cannot satisfy the two specific conditions at the same time, The tested product is identified as containing no potato starch. The fluorescence quantitative PCR technology provided by the invention has the characteristics of fast, simple, accurate, good specificity and high sensitivity for the detection of adulteration in starch and products, and provides a powerful means for food inspection and analysis.
priorityDate 2019-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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