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filingDate 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110872344-B
titleOfInvention Chloramphenicol complete antigen and preparation method and application thereof
abstract The invention discloses a chloramphenicol complete antigen and a preparation method and application thereof. The chloramphenicol complete antigen is a conjugate of hapten and carrier protein; wherein, the structural formula of the hapten is shown as the following formula I: the preparation method comprises the following steps: synthesizing the hapten; and then activating the hapten, and coupling the hapten with the carrier protein to obtain the chloramphenicol complete antigen. A chloramphenicol antibody which is produced by using the above chloramphenicol complete antigen. The application of the chloramphenicol complete antigen or the chloramphenicol antibody of the invention in the following 1) or 2): 1) detecting chloramphenicol in the sample; 2) preparing at least one of an enzyme linked immunosorbent assay kit, a test strip and an immunoaffinity chromatography column for detecting chloramphenicol in a sample; wherein the sample is a water body and/or a food. The chloramphenicol complete antigen can be used for preparing the chloramphenicol complete antigen from the alpaca antibody, and conditions are laid for further screening high-quality chloramphenicol nano antibodies.
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