| abstract |
The present invention provides specific LAMP primers, kits and methods for detecting Staphylococcus aureus. LAMP primers include the following 3 pairs of primers: outer primers F3 and B3, inner primers FIP and BIP, and loop primers LB and LF, 6 loop-mediated isothermal amplification primers are designed for the conserved sequence of Staphylococcus aureus KX‑371871.1 gene; FIP The nucleotide sequence of F3 is shown in SEQ.ID.NO.1, the nucleotide sequence of BIP is shown in SEQ.ID.NO.2, and the nucleotide sequence of F3 is shown in SEQ.ID.NO.3, The nucleotide sequence of B3 is shown in SEQ.ID.NO.4, the nucleotide sequence of LB is shown in SEQ.ID.NO.5, and the nucleotide sequence of LF is shown in SEQ.ID.NO.6 . The invention provides a more rapid and portable method for the detection of Staphylococcus aureus. |