http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110862978-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 |
| filingDate | 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110862978-B |
| titleOfInvention | A kind of preparation method of recombinant halophilic archaeal protease |
| abstract | The invention belongs to the field of bioengineering, in particular to a method for preparing a recombinant halophilic archaeal protease; the steps are as follows: using the genome of Halostella sp.DL-M4 as a template and pET28a plasmid as an expression vector to construct a recombinant plasmid; transforming the recombinant plasmid into Escherichia coli The cells were induced to express, the cells were collected by centrifugation for cell resuspension and fragmentation, the supernatant was obtained by centrifugation again, and β-mercaptoethanol was added to obtain a mixed solution, which was passed through a nickel column, the zymogen was combined with the nickel medium and renatured, and finally the second impurity removal was carried out. Elution of protein and target protein, ultrafiltration, centrifugal concentration, and gel filtration chromatography to obtain recombinant halophilic archaeal protease; the invention has good versatility, simpler purification steps, shorter production cycle, and high purity of the obtained protease. The halophilic archaeal protease with a specific enzyme activity of 1159.15 U/mg was finally obtained. |
| priorityDate | 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 276.