| abstract |
The invention discloses a method for in vitro culture and amplification of NK cells by gallic acid, which can establish stable and large-scale high-purity and high-immunocompetence NK cells without serum, feeder cell stimulation and pre-coating of a culture bottle, and is simple and feasible; gallic acid and IL-2 are used together for stimulating NK cell expansion culture, and can reduce T cell number, improve NK cell purity and enhance NK cell activity, and can make CD56 with immunoregulation function in the culture method high The CD16-NK cell subset proportion is increased, so the culture method is expandedThe NK cell product of (1) can be emphatically applied to the medicines of immunomodulators, and the culture method does not reduce the activity and the quantity of NK cells. |