http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110862945-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110862945-B |
| titleOfInvention | A kind of fermentation method of Escherichia coli induced protein expression |
| abstract | The invention discloses a fermentation method for Escherichia coli to induce protein expression, and belongs to the field of biotechnology. The fermentation method comprises the following steps: inoculating the seed liquid to be fermented in the fermentation culture liquid, and placing it under the pressure condition of 0.05-0.1 MPa to carry out constant-temperature fermentation and culture for 6-10 hours to obtain the fermentation bottom liquid; to the above-mentioned fermentation bottom liquid Add an inducer; the mass concentration of the inducer in the fermentation bottom liquid is 0.1 ~ 1.5mmol/L; add feed liquid to the above fermentation bottom liquid with inducer, and continue to carry out constant temperature fermentation and cultivation for 20 ~ After 48 hours, the fermentation product was obtained; the volume ratio of the feeding liquid to the fermentation bottom liquid with the inducer added was (0.5-2):1. The fermentation method provided by the present invention can induce protein expression in an environment of 36-40°C. It does not need to rapidly cool down the fermentation bottom liquid, and can also reach the protein expression level when the temperature is lowered to 25-30°C, thereby greatly reducing energy consumption and save production costs. |
| priorityDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.