http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110849863-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-658 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-65 |
| filingDate | 2019-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110849863-B |
| titleOfInvention | Method for detecting conformational change in binding process of aptamer and ligand small molecule |
| abstract | The invention relates to the technical field of biological medicines, in particular to a method for quickly and effectively detecting conformational change in the process of combining an aptamer and a ligand small molecule, which comprises the following steps: firstly, determining the affinity between the selected aptamer and the small molecule by using a biological membrane interference experiment; then detecting the conformational change before and after the aptamer is combined with the small molecule by using a surface enhanced Raman scattering spectroscopy technology; and finally, intuitively explaining the binding process of the aptamer and the ligand through molecular dynamics simulation. The invention takes theophylline and an aptamer thereof as an example, verifies the reliability of the method for researching the conformation change of the aptamer, provides a new method and a new thought for designing, modifying and screening the aptamer with high affinity and high selectivity, and leads the aptamer to be better applied to the fields of detection, sensing, clinical diagnosis, treatment and the like. |
| priorityDate | 2019-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.