http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110846286-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-18851 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 |
| filingDate | 2019-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110846286-B |
| titleOfInvention | Method for labeling Sendai virus envelope with rare earth up-conversion fluorescent particles |
| abstract | The invention discloses a method for marking the envelope of Sendai virus by using rare earth up-converting fluorescent particles, comprising the following steps: comprising biotin ligase (BirA), biotin binding site (APtag) and glycosylated phosphatidylinositol cell membrane The construction of the high-efficiency expression plasmid pCDH-BirA-AP-GPI of the anchor sequence (GPI); the synthesis of streptavidinized rare earth up-conversion fluorescent particles; the preparation of envelope biotinylated Sendai virus; the use of streptavidin Biotinylated Sendai Virus Enveloped Biotinylated Sendai Virus Labeled with Rare Earth Upconverting Fluorescent Particles. The virus envelope labeling technology of the present invention can achieve a virus labeling efficiency of 40% to 60%. Under the excitation of 980nm near-infrared light, the rare earth up-conversion fluorescent particles used can emit green fluorescence with a wavelength of 540nm, which can be observed under a confocal microscope . |
| priorityDate | 2019-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.