http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110846275-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_98496d20aeb4ee836714e3dbbd0785d8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-135 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0667 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2019-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cba1e102f43d2769baaa3b66070d28df |
| publicationDate | 2020-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110846275-A |
| titleOfInvention | A serum-free culture method for human autologous adipose-derived mesenchymal stem cells |
| abstract | The invention relates to a serum-free culture method for human autologous adipose mesenchymal stem cells, which specifically includes selecting sterile adipose tissue for first digestion to obtain a first cell suspension; and performing second digestion on undigested adipose tissue to obtain The second cell suspension; the first cell suspension and the second cell suspension are combined and then cultured, passaged and identified to obtain the human autologous adipose mesenchymal stem cells. In the adipose-derived mesenchymal stem cell culture method of the present invention, the separation, expansion culture and cryopreservation systems of primary cells are all serum-free medium, which completely avoids the risk of adding animal-derived components; the method of the present invention adopts a two-step enzyme The digestion method uses a specific ratio of digestive enzymes to adipose tissue to fully contact the adipose tissue with the enzyme solution, so that the adipose tissue can be fully digested without damaging the cells. The yield of primary cells is high, the activity is good, and the repeatability is high. , good safety, in line with clinical application standards. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111763656-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111944748-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111944749-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115521909-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522193-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112522193-B |
| priorityDate | 2019-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 136.