http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110845593-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N23-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43504 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N23-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
| filingDate | 2019-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110845593-B |
| titleOfInvention | Separation and identification method of patinopecten yessoensis myofibrils |
| abstract | The invention relates to a separation and identification method of comb shell myofibrils, belonging to the field of muscle biology of marine invertebrates, which comprises the following steps of washing the adductor muscle tissue with a special washing liquid for the scallop muscle, adding a relaxation buffer solution for the scallop muscle, and rotating and stirring for 3 days; cutting the adductor muscle tissue, adding scallop muscle relaxation buffer solution, and homogenizing until the tissue mass completely disappears; centrifuging at 4 deg.C, adding scallop muscle relaxation buffer solution into the supernatant, centrifuging at 4 deg.C to obtain precipitate of coarse myofilament, rapidly blowing with scallop muscle relaxation buffer solution, immediately vortex and mixing, and centrifuging to obtain supernatant; adding scallop muscle relaxation buffer solution into the supernatant, centrifuging to obtain precipitate of the coarse myofilaments, blowing and uniformly mixing with the scallop muscle relaxation buffer solution, centrifuging to obtain supernatant which is purified coarse myofilament suspension and can be directly used for SDS-PAGE detection and electron microscope analysis; the method can realize the high-efficiency separation of the myofibrils of the adductor muscles of the patinopecten yessoensis. |
| priorityDate | 2019-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.