http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110845569-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_311159e9645e7b312c4272609ac1e08d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2019-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fbcb5a97f18fd0c9818d55706dfed6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_435ace50a1fbd69b868ac8d7517c6c78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3acbb6e4b421dfc4a64dd84fbab5ef86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f919ee4f06436998c5cbdbbbc66af63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d0ebdb0c5b6b060a83c53d50692c2e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8d676202a530502a0ca24e686787c9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad0585e19b989f259c81e5f9f870fecb |
| publicationDate | 2020-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110845569-A |
| titleOfInvention | A method for extracting total protein from Echinococcus granulosus |
| abstract | The invention discloses a method for extracting total protein from Echinococcus granulosus, comprising the following steps: (1) preparing a lysate according to RIPA:PMSF=100:1; (2) removing the Echinococcus granulosus from an incubator Take out a pre-cooled 2ml centrifuge tube, transfer the Echinococcus granulosa into the centrifuge tube and centrifuge, discard the supernatant after centrifugation, absorb PBS after centrifugation and add lysate to each group, put it in the refrigerator for lysing, After the shaker was slightly shaken, it was reset to the refrigerator for lysis, shaken once every 5 minutes, and pulverized in a cell ultrasonic disintegrator; (3) centrifuged in a low-temperature high-speed centrifuge; (4) sucked the supernatant to another pre-cooled labeled cell. In the 2mL centrifuge tube, it is the total cell protein obtained by extraction. The invention can extract a higher concentration of protein from the same amount of Echinococcus granulosus, which provides a guarantee for further experiments. |
| priorityDate | 2019-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.