http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110835630-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-04001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110835630-B |
| titleOfInvention | A highly efficient sgRNA and its application in gene editing |
| abstract | The invention provides a highly efficient sgRNA and its application in gene editing. The sgRNA is shown in formula I: the RNA-transformed sgRNA backbone (formula I) of the target sequence transcription; the modified sgRNA backbone is to insert RNA fragment A between the 14-15 positions of the sgRNA backbone, and The RNA molecule obtained after inserting RNA fragment B between positions 18-19 of the sgRNA backbone; the RNA fragment A and the RNA fragment B are reverse complementary; the RNA fragment A and the RNA fragment B have the same size 3nt; the sgRNA backbone is the RNA molecule shown in Sequence 9. It is proved by experiments that the transformed sgRNA of the present invention can significantly improve the C·T base replacement efficiency of a cytosine base editor (CBE), up to 86.4%. |
| priorityDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 955.