| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_abd156263f0d78fc96518fafc94f377f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-04001 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 |
| filingDate |
2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_472cc2bbe36fb9bd5a9e92667b8f104a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b59fe026e5031fbe29e0ef4205bd1d4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66047a3480290c929f666cd0ba092028
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10909c7f5546a85db4db85b84b355aa0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b8a1844ad008bb0279130a0af338b87 |
| publicationDate |
2020-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110835630-A |
| titleOfInvention |
A high-efficiency sgRNA and its application in gene editing |
| abstract |
The present invention provides an efficient sgRNA and its application in gene editing. Described sgRNA is as shown in formula 1: the sgRNA backbone (formula 1) of the RNA-transformation of described target sequence transcription; The sgRNA backbone of described transformation is to insert RNA fragment A between the 14-15th positions of the sgRNA backbone, and The RNA molecule obtained by inserting RNA fragment B between positions 18-19 of the sgRNA backbone; the RNA fragment A and the RNA fragment B are inversely complementary; the RNA fragment A and the RNA fragment B have the same size is 3nt; the sgRNA backbone is the RNA molecule shown in sequence 9. It is proved by experiments that the modified sgRNA of the present invention can significantly improve the C·T base substitution efficiency of the cytosine base editor (CBE), up to 86.4%. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023005935-A1 |
| priorityDate |
2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |