| abstract |
The invention provides a tissue culture method for flower sticks. The growth points on the young shoots of flower sticks are taken as explants, and after sterilization, primary induction culture, subculture, rooting culture and transplanting are carried out. Selection of explants of flower sticks, optimal culture conditions, optimal medium at different stages and proliferation culture generation. Through experiments, the best medium and medium combination at different stages in the tissue culture of flower sticks were obtained, and the optimum culture conditions and culture methods were obtained, which solved the problems of vitrification of flower stick tissue culture, shortening the seedling time, and provenance in cultivation. The problem of less problems has laid a reliable foundation for the factory production of flower sticks. The invention has the advantages of simple operation, low cost and good application prospect. |