abstract |
The invention discloses a PirB gene knockout mouse animal model and a construction method thereof, which are obtained by the following method: based on CRISPR/Cas9 technology, the specific target sites gRNA1 and gRNA2 of the C57BL/6J mouse PirB gene to be knocked out are respectively combined with The trancrRNA was incubated at 25°C for 10 min to form a hairpin structure; the active gRNA1, gRNA2, Cas9 enzymes and the prepared targeting vector containing cKO region, homology arm and loxP site were microinjected into the fertilized eggs of surrogate mice to obtain F0 Generation of mice, and then to obtain F1 generation heterozygous mice and F2 generation homozygous mice in turn, to obtain the PirB gene knockout mouse model of the present invention. The invention provides a good foundation for the research and in vivo verification of the PirB gene function, and especially has high research value for the research on AD phenotype and pathological symptoms after PirB is knocked out. |