http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110804559-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P37-00 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-82 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate | 2019-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110804559-B |
| titleOfInvention | Recombinant penicillium chrysogenum gene engineering bacterium and construction method and application thereof |
| abstract | The patent discloses a recombinant penicillium chrysogenum genetic engineering bacterium and a construction method and application thereof, wherein a sequence of a Pc-somA gene in the penicillium chrysogenum genetic engineering bacterium is replaced by a Bleo resistance gene; the penicillium chrysogenum is ATCC 48271; the construction method comprises the following steps: (1) extracting genome DNA of penicillium chrysogenum; (2) constructing a gene knockout fragment; (3) preparing protoplasts of penicillium chrysogenum; (4) and (3) introducing the gene knockout fragment into the prepared protoplast for homologous recombination to obtain the recombinant penicillium chrysogenum genetic engineering bacterium. Has the advantages that: the genetic engineering bacteria provided by the invention increase the biofilm amount OD570 of penicillium chrysogenum in the immobilized fermentation penicillin production process by nearly 2 times, so that hypha can be better adsorbed and combined with a plastic medium, the titer of penicillin is improved to 1963.25U/mL, the whole fermentation period is shortened, 24h is shortened, and the production cost is reduced. |
| priorityDate | 2019-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.