http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110791543-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e89c206df73c7928c3fa05f87f1287de |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
filingDate | 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f6834962dbd2eea34f0f73afeb6dfe8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_550fab7a631b89b92be01f763e3d0b9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca1d5ab8d423de9ad2b848b73d1f5089 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bd045f7703328092292666771e01b21 |
publicationDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110791543-A |
titleOfInvention | A kind of identification method of natural product drug action target |
abstract | The invention provides a method for identifying the target of natural product medicine. The present invention includes the following steps: 1) adding the drug to be tested and the control solvent to the effective cells respectively, and incubating together; 2) placing at 30-80° C., heat-treating for 3-10 min, lysing, and centrifuging to obtain a supernatant; 3. ) Electrophoresis, transfer; 4) Use broad-spectrum protein antibodies or dyes to stain respectively, and compare, to find the bands with differences in protein stability; 5) Take another sample of the drug to be tested by electrophoresis, and analyze the bands with differences in protein stability by mass spectrometry , Verify, get the target. The invention is a natural product drug target identification method based on protein thermal stability and broad-spectrum molecular staining, which can identify potential drug targets with high efficiency, low cost and label-free, and the amount of natural products is small, and potential drug targets can be found. Drug targets can not only drive disease mechanism of action and pharmacology research, but also provide guidance information for potential side effects of drugs and commercialization of drugs. |
priorityDate | 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.