http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110791474-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_663220a8ce750d8aa0fc75dff341b727 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0669 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2019-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8c87e62901512b3bab440c0792d1719 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6bf8f3c263fc4611096b3cf9e552328 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d801fd1be1f4aaa345c38250b4e769b6 |
| publicationDate | 2020-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110791474-A |
| titleOfInvention | A kind of isolation and in vitro culture method of nucleus pulposus cells |
| abstract | The invention discloses a method for separating and culturing nucleus pulposus cells in vitro. When the nucleus pulposus cells are separated, blood stains and cartilage tissue should be cleaned up to obtain nucleus pulposus tissue, so as to prevent miscellaneous cells from affecting the adherence of nucleus pulposus cells and subsequent culture, and the digestive juice With collagenase I, the digestion process is milder, the cells and tissues after digestion are more active, and they are easy to adhere to the wall. The digestion is terminated with a mixture containing 20% fetal bovine serum and DPBS, and the obtained cells are not easy to adhere. The bottle is more uniform, and the reagents used are added with 1% penicillin/streptomycin to prevent the contamination of the sample itself or during collection and transportation; in vitro culture, add the placental mesenchymal stem cell culture supernatant lyophilized powder solution, the addition amount is 10-40 %, make up for the lack of growth factors in the medium, and promote the growth of nucleus pulposus cells; the cultured cells have a high survival rate, are easy to adhere to, and have good cell morphology and high and stable quality. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112061600-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113025567-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112920992-A |
| priorityDate | 2019-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.