| abstract |
The invention discloses a method for synthesizing diglyceride. After mixing a fatty acid donor, glycerol, partial glyceride lipase and monoglyceride lipase with water, an esterification reaction is carried out, and the reaction time is 8-24h, and further separation and purification are performed. Diglycerides are obtained. The present invention utilizes monoglyceride lipase to promote the reaction efficiency of partial glyceride lipase in the esterification reaction, so as to improve the rate of synthesizing diglyceride. Compared with single enzyme, the synthesis time is shortened by more than half, and after the esterification reaction More than 45.50% of diglycerides are obtained, because the product basically does not generate triglycerides, after molecular distillation and purification, the DAG content is as high as more than 98%. The invention is a fast, efficient, green and convenient for industrialized synthesis method. |