http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110760608-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2019-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110760608-B |
| titleOfInvention | Rape BnFAD2 gene high oleic acid allelic mutation and development and application of SNP marker primer thereof |
| abstract | The invention relates to a novel high oleic acid allelic mutation of rape BnFAD2 gene, and relates to the development of a novel high oleic acid allelic variation SNP molecular marker and a molecular marker-assisted breeding technology. The invention discloses two pairs of novel high oleic acid allelic mutations (BnAFad 2a and BnCfad 2a) of a BnFAD2 gene in brassica napus. The nucleotide sequence of the gene is shown as SEQ ID NO: 1 and ID NO: 2, the preparation method is as follows. The invention also provides two pairs of special KASP molecular markers KASP-421 and KASP-1073 for rapidly screening the novel high oleic acid allelic mutation sites, and the markers are used for single plant genotype identification and high oleic acid strain screening. The two pairs of KASP molecular markers provided by the invention are developed by SNP variation of rape BnFAD2 gene exon regions, the specificity of the screening result is higher, and the detection result is more accurate and reliable. The KASP molecular marker provided by the invention only needs two steps of PCR and fluorescence detection, has low cost and high flux, and is particularly suitable for classified screening and identification of offspring high oleic acid genotypes of breeding groups. The combination of the two molecular markers can screen the progeny material with the oleic acid content of up to 88%. |
| priorityDate | 2019-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 359.