| abstract |
The present invention relates to a real-time fluorescent RPA detection kit of Aspergillus fumigatus and special primers and probes thereof. Specifically, the present invention proposes a primer composition for RPA detection, the primer composition comprising: a forward primer, the forward The primer has the nucleotide sequence shown in SEQ ID NO: 1; the reverse primer has the nucleotide sequence shown in SEQ ID NO: 2. The primer composition detected by the RPA can quickly, conveniently, efficiently, specifically and sensitively detect the Aspergillus fumigatus components in the samples waiting to be tested in bacterial cells, sputum, blood, and bronchoalveolar lavage fluid under isothermal conditions. |