abstract |
The invention relates to the field of biotechnology, in particular to the application of serum exosome ssc-miR-17-5p as a molecular marker for early pregnancy diagnosis of sows. The nucleotide sequence of the serum exosome ssc-miR-17-5p is shown in SEQ ID No.1. The use of this molecular marker to detect pregnancy status has the characteristics of early, convenient, rapid, specific and sensitive, thereby shortening the empty pregnancy period of pseudo-pregnant sows, and adopting compounding as soon as possible is beneficial to production management and improves production efficiency. The invention also provides a primer and a kit for detecting the above-mentioned molecular marker, which provides a new way for the early pregnancy diagnosis of sows. |