http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110747258-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d72888b637c9b60db0bc5d390090a5df |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2018-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e8b177ae4171d4cf95479f471c0424e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc8f55fa9db7dc590665da1bd1ff7ff2 |
| publicationDate | 2020-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110747258-A |
| titleOfInvention | Oil phase composition for preparing droplets in droplet digital PCR and application thereof |
| abstract | The invention relates to the technical field of biology, and discloses an oil phase composition for preparing droplets in a droplet type digital PCR (polymerase chain reaction) and application thereof. The oil phase composition comprises an oil phase 1, an oil phase 2, an oil phase 3 and a surfactant 1; wherein the oil phase 1 is mineral oil or alkane, the oil phase 2 is alkane or ester oil, the oil phase 3 is alkane, and more than two of the oil phases 1-3 are different from each other when being alkane; the surfactant 1 is a silicone-based water-in-oil active agent. The invention combines specific oil phase and surfactant to form an oil phase composition with excellent performance, so that the oil phase composition can enable formed liquid drops to be more uniform and have smaller deviation when being applied to liquid drop type digital PCR, and can fully amplify a target sequence to be closer to the initial copy number of the target sequence, thereby enabling the PCR result to be more accurate. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113136421-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113136421-B |
| priorityDate | 2018-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.