http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110747208-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y107-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8281 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2019-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110747208-B |
| titleOfInvention | Cassava nitrate reductase gene and construction and disease-resistant application of overexpression vector thereof |
| abstract | The invention provides a cassava nitrate reductase gene and construction and disease-resistant application of an overexpression vector thereof, belonging to the technical field of plant genetic engineering. The invention separates and obtains a gene from cassava, and the nucleotide sequence of CDS is shown in SEQ ID No. 1. Construction of cassava MeNR1 gene overexpression vector: extracting RNA from No. 124 cassava leaves in south China, carrying out reverse transcription to obtain cDNA, taking the full-length gene sequence of CDS region of MeNR1 gene as gene over-expression vector insert fragment, selecting XbaI and SmaI enzyme cutting sites to design pBI121 homologous primer, taking the obtained cDNA as a template to carry out amplification to obtain the full-length sequence of MeNR1 gene coding region, cutting gel, recovering, and connecting to plant over-expression vector pBI121 through homology to obtain pBI121-MeNR1 gene over-expression vector. An effective transient MeNR1 gene overexpression plant is obtained by constructing an overexpression vector of the MeNR1 gene and injecting cassava leaves, and a quick and effective method is provided for researching the cassava gene function. |
| priorityDate | 2019-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.