http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110747195-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1138 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-713 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110747195-B |
| titleOfInvention | shRNA and lentiviral vector for inhibiting human EDRADD gene expression as well as construction method and application thereof |
| abstract | The invention discloses shRNA for inhibiting the expression of human EDRADD genes, a lentiviral vector, a construction method and application thereof, wherein the method comprises the steps of firstly synthesizing a positive strand and a negative strand of a DNA oligo aiming at an RNA interference target sequence; annealing the positive strand and the reverse strand to form double-stranded DNA with a sticky end; linearizing a lentivirus vector by enzyme digestion, and connecting the linearized vector with double-stranded DNA; transforming the ligation product into an escherichia coli competent cell, and performing PCR identification on the obtained positive clone; plasmid extraction is used for the next step of virus packaging. According to the invention, an RNA interference target sequence is designed by taking an EDRADD gene as a template, an shRNA interference sequence is designed according to the selected target sequence, an RNA interference lentiviral vector is constructed by packaging, and after the EDRADD gene is knocked down, the proliferation of a tongue squamous cell carcinoma CAL-27 cell is obviously influenced, the cell clone forming capability is influenced, and the apoptosis of the cell is promoted. |
| priorityDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.