http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110734962-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110734962-B |
| titleOfInvention | Method for detecting food toxin based on aptamer |
| abstract | The invention discloses a method for detecting food toxin based on a nucleic acid aptamer, which comprises the following steps: 1) cleaning the glass slide with the groove, and then performing amino silanization and aldehyde modification on the surface of the glass slide; 2) fixing capture probe capture DNA on an aldehyde glass; 3) adding NPS-aptamer, dripping 10.0 μ L3 XSSC buffer solution, and placing it into a shaking incubator to hybridize for 25-40min at 30-45 deg.C; 4) adding food toxin solution into the glass groove, and reacting for 20min at 45 ℃; 5) the reaction solution was transferred to a PCR tube, and H was added 2 O 2 And TMB solution, irradiating for 10s-25min under 808nm wavelength, and measuring the temperature change of the solution with a thermometer; 6) and establishing a fitting curve graph of the temperature change and the food toxin adding amount to realize the determination of the food toxin. The method has the advantages of simple and convenient operation, good selectivity and no need of expensive instruments. |
| priorityDate | 2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.