http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110734961-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 |
filingDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110734961-B |
titleOfInvention | Enzyme-free biosensor for detecting activity of uracil-DNA glycosylase |
abstract | The invention provides an enzyme-free biosensor for detecting uracil-DNA glycosylase, which comprises a UDG template chain 1, a silver nanocluster DNA template chain 2 and a DNA chain 3 rich in cytosine. The invention adopts a fluorescence method to detect the activity of uracil-DNA glycosylase, the excitation wavelength of fluorescence detection is 570nm, the emission wavelength is 630nm, and the detection waveband is 590-720 nm. The biosensor has the advantages of good specificity, high sensitivity, mild reaction conditions and high reaction speed; because no external enzyme is added in the reaction process and silver nanocluster fluorescence detection is adopted, the method is simple and convenient to operate, low in reaction requirement, short in detection period, stable in performance and good in repeatability, and is suitable for detecting the UDG in the field of medical treatment and health. |
priorityDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.