http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110724735-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6879 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6879 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110724735-B |
| titleOfInvention | A kind of SNP site, primer and method for rapid identification of individual sex of pufferfish |
| abstract | The invention discloses a SNP site, primers and a method for rapidly identifying the individual sex of pufferfish with dark stripes. The base sequence of the SNP site is shown in SEQ ID NO.1. Genome analysis of male and female individuals in independent genetic groups obtained a male-and-male-specific SNP locus, and two pairs of male-specific PCR primers were designed for this locus to identify the male and female sex of pufferfish individuals; improved DNA extraction The method can complete the extraction of fin ray DNA within 10 minutes, which is simple and fast. Then, through PCR amplification and agarose gel electrophoresis, male individuals with DNA bands are identified as male individuals, and vice versa, female individuals are easily distinguished. The invention has the characteristics of high speed, high accuracy, simple operation, low cost and the like, and can complete the identification of the genetic sex of pufferfish with dark stripes within 2 hours by using the method. |
| priorityDate | 2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.