| abstract |
The invention provides a method for targeted editing of ISS sequences by CRISPR recombinant plasmids constructed from nucleotide sequences. The steps are as follows: target design of sgRNAqingqin oligo sequences, addition of BsmBI plasmid restriction sites at both ends, construction of recombinant plasmids, transformation, and plating, Single clones were picked, plasmids were extracted, sequenced, sequence aligned, and the construction was successful after identification. The present invention adopts the CRISPR case 9 sgRNAqingqin recombinant plasmid, which can precisely target editing (including deletion, insertion and mutation) ISS sequence, and can also achieve the effect of making the splicing silencer ISS sequence ineffective or weakened, thereby significantly promoting the SMN2 exon 7 is included, and the recombinant plasmid can play a role in continuous expression in cells, and one-time transfection is sufficient, which is the most potential method for the treatment of related genetic diseases. |