http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110713934-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-32 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-26 |
| filingDate | 2019-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110713934-B |
| titleOfInvention | Edible fungus production strain preservation method and activation method thereof |
| abstract | The invention discloses a preservation method and an activation method of edible fungus production strains, wherein the preservation method comprises the following steps: (1) placing the mixture (glycerol 30-50% (v/v) and paraffin oil 50-70% (v/v)) in a sealed container, and sterilizing the mixture; (2) selecting a flat plate without lacking of a corner of a bacterial colony, selecting tip hyphae to culture on a culture medium slope with poor nutrition, adding a sterile mixed liquid into a test tube to cover the culture medium slope and the hyphae when the culture grows to 4/5 times of the length of the culture medium slope, and preserving at normal temperature; the activation method comprises the following steps: (1) adding 10-40% trehalose solution into solid culture medium, and sterilizing; (2) picking up 1cm multiplied by 1cm fungus blocks by using an inoculation shovel after burning and cooling, transferring the fungus blocks into a fresh sterile solid culture medium for culture, transferring the fungus blocks into a seed bag and a production culture bag after flat hyphae grow stably, and putting the fungus blocks into production and use after no abnormal phenomenon occurs; the method can be preserved at normal temperature for a long time (3-5 years), the subsequent preservation is not influenced when the method is used midway, the defects of the traditional method are overcome, and the original activity of the strain is maintained. |
| priorityDate | 2019-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.