| abstract |
The invention belongs to the technical field of medicine, and relates to a set of in vitro dynamic drug metabolism incubation system, which is composed of an incubation system with drug metabolism enzymes, a constant temperature device, a peristaltic pump, an effector cell system and a sampling system, and performs serial circulatory perfusion; wherein , the incubation system uses a hydrogel with a temperature-sensitive 3D structure as a carrier to encapsulate microsomes, recombinases or hepatocytes with metabolic capabilities, plus reaction substances such as initiators; the sampling system consists of a semi-permeable membrane The dialysis system is constructed to perform real-time free state sampling. The system fully considers the entire process of drug diffusion, distribution and transport in the system, binding and reaction with metabolic enzymes, and ultimately affecting effector cells in a dynamic environment. The invention is suitable for estimating the pharmacokinetic parameters of the drug in the body, the clearance rate CL and the inhibition rate constant Ki, Kinact, KI, etc., and truly reflect the pharmacokinetics (PK), drug interaction (DDI) and drug Effectiveness (PD). |