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filingDate 2019-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2e14a44116811e952de0487ed7d081e
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publicationDate 2020-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110699466-A
titleOfInvention A kind of SNP locus associated with resistance to Vibrio eel in large yellow croaker, its screening method and application
abstract The invention belongs to the technical field of fish molecular marker screening, and specifically discloses a SNP site associated with the large yellow croaker against Vibrio eel, a screening method and application thereof, and the SNP site is located in the large yellow croaker as shown in SEQ ID NO: 1 On the IL-6R gene cDNA sequence, and there is an allelic mutation of G or C at the 1021 base; after extracting the RNA of Vibrio eel infecting large yellow croaker juveniles and reverse transcribing into cDNA, the design is as SEQ ID NO The forward primer described in: 2 and the reverse primer shown in SEQ ID NO: 3 are obtained by performing PCR amplification reaction with cDNA as a template, and performing sequencing and screening on the PCR product with a clear target band and no stray bands. The SNP site related to the anti-Vibrio eel in the invention can be used for the genetic breeding of the large yellow croaker with resistance to Vibrio eel, and provides a new idea for the antibacterial breeding work of the large yellow croaker.
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