http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110699322-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08G81-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08G81-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-08 |
| filingDate | 2019-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110699322-B |
| titleOfInvention | Three-dimensional tumor cell culture medium and preparation method thereof |
| abstract | A three-dimensional culture medium for tumor cells is prepared by the following steps: dissolving modified chitosan in distilled water of 30-35 deg.C, adjusting pH to 7-8, performing ultrasonic treatment for 10-24h, adding cross-linking agent, initiator and ethanol in proportion, stirring for reaction for 30-60min, stopping stirring, and continuing to react at 50-60 deg.C for 2-5h to obtain culture medium; the modified chitosan is prepared by mixing a chitosan solution and an N-isopropyl acrylamide or N, N-methylene bisacrylamide solution according to a molar ratio of 1.5-5:1, adjusting the pH value to 3-6, reacting at 25-35 ℃ for 1-10h to obtain a modified chitosan solution, dehydrating and drying the modified chitosan solution at 60-80 ℃ in vacuum, and cleaning with deionized water. The culture medium has good biocompatibility, no toxic or side effect on cells, good mechanical properties, and is convenient for animal cell experiment xenotransplantation and subsequent analysis of the cells. |
| priorityDate | 2019-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.