http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110698548-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 |
| filingDate | 2019-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110698548-B |
| titleOfInvention | Cordyceps militaris active protein CMPr and preparation method and application thereof |
| abstract | The invention discloses a cordyceps militaris active protein CMPr, and a preparation method and application thereof, wherein the preparation method comprises the following steps: s10, dissolving the total protein of the cordyceps militaris sporocarp by using PBS buffer solution to enable the protein concentration to be 15-20 mg/mL; s20, taking 2-3 mL of cordyceps militaris sporocarp total protein solution, filtering, loading the filtrate to a molecular sieve Sephadex G-50 chromatographic column, and eluting with PBS buffer solution to obtain a first elution map; step S30, collecting components containing Cordyceps militaris active protein CMPr according to the first elution map; step S40, concentrating the component containing the cordyceps militaris active protein CMPr until the protein concentration is 15-20 mg/mL; s50, loading 2-3 mL of concentrated solution into a Superdex 75 gel chromatography column, eluting with PBS buffer solution to obtain a second elution pattern, and collecting pure Cordyceps militaris active protein CMPr according to the second elution pattern. The invention aims to separate a single protein CMPr with immune activation activity from cordyceps militaris sporocarp. |
| priorityDate | 2019-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.