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filingDate 2019-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110684819-A
titleOfInvention A screening method for effective fungicides for sugarcane tip rot pathogenic bacteria
abstract The invention discloses a screening method for an effective bactericide for sugarcane tip rot pathogenic bacteria. First, the sugarcane tip rot pathogenic strain is activated on a PDA medium; then the bactericide is prepared as a mother solution, which is then diluted into a concentration gradient medicinal solution, and 2 ml of The medicinal liquids of different dilution concentrations were added to 98ml PDA medium respectively, and after mixing, they were divided into petri dishes, and after cooling, the medicine-containing plate was obtained, and the medicine-free plate was used as a control; Take the bacterial cake from the edge of the rot pathogenic bacteria colony, transfer the bacterial cake to the center of the drug-containing plate, and place it in a 28°C constant temperature incubator for cultivation; after the cultivation is completed, draw a line on the bottom of the plate with the bacterial cake as the center to measure the diameter of the colony. The inhibition rate of the fungicide was determined, and the EC 50 was calculated; then the fungicide or the compound fungicide with EC 50 ≤ 30 mg/L was screened out for field control experiments. The invention realizes the rapid and effective screening of bactericides for sugarcane tip rot pathogenic bacteria under in vitro conditions, and has the advantages of short screening period, simple operation and low cost.
priorityDate 2019-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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