http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110684796-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-11012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-10 |
| filingDate | 2019-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110684796-B |
| titleOfInvention | Method for specifically knocking out soybean lipoxygenase gene by CRISPR-Cas9 and application thereof |
| abstract | The invention discloses a method for specifically knocking out a soybean lipoxygenase gene by CRISPR-Cas9 and application thereof, belonging to the field of soybean molecular breeding and biotechnology. The method comprises the following steps: targeting of highly homologous soybean lipoxygenase gene coding region design GmLox1 And GmLox2 sgRNA of (a) GmLox1/2， And targeting GmLox3 sgRNA of (a) GmLox3 (ii) a Constructing a CRISPR-Cas9 gene knockout vector for each sgRNA independently, and then infecting soybean cotyledonary nodes in a mixed manner; stably genetically transforming soybean to obtain plant Gmlox‑28 And Gmlox‑60 knock out 3 simultaneously GmLox A gene; after selfing for two generations, at T 2 5T-DNA strains separated from editing sites in generation plants GmLox All deletion mutants. The method provides a new strategy for directly and purposefully and rapidly creating a new soybean germplasm without beany flavor. |
| priorityDate | 2019-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.