| abstract |
The invention relates to the technical field of chemical detection and analysis, in particular to a method for detecting plant secondary metabolites based on SWATH to MRM technology. A method for detecting plant secondary metabolites based on SWATH to MRM technology comprises the following steps: A. sample treatment: adding 25-100mg of ground plant sample into a centrifuge tube, adding 500-1500 mu L of 75% methanol, mixing, centrifuging, and taking supernatant; B. and (3) computer detection: b, setting conditions of liquid chromatography and mass spectrum, and carrying out on-machine detection on the supernatant obtained in the step A; C. data processing: and C, processing and analyzing the data obtained by the computer detection in the step B. The method further improves the flux and accuracy of the detection method by utilizing the mutual synergistic effect of various parameters, weakens the matrix effect, is more favorable for comprehensively and accurately analyzing the metabolites, and can be suitable for detecting and analyzing the secondary metabolites contained in any tissue organ of any plant. |