http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110669709-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2019-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110669709-B |
| titleOfInvention | Method for efficiently synthesizing PHB (polyhydroxybutyrate) by knocking out flagellum and pilus genes |
| abstract | The invention discloses a method for efficiently synthesizing PHB by knocking out flagella and pilus genes, belonging to the field of genetic engineering and fermentation engineering. According to the invention, three flagella gene clusters flhE-motA, fliY-fliR and flgN-flgL on an escherichia coli genome are knocked out to obtain a flagella reduced strain WJW010, and a pilus gene cluster fimB-fimH is knocked out to obtain a pilus reduced strain WJW 011. Then, three genes synthesized by the PHB are transformed into strains WJW010 and WJW011 to obtain recombinant strains WJW010/pBHR68 and WJW011/pBHR68, the PHB with the cell dry weight of 19.2 percent and 2.8 percent can be synthesized under normal fermentation conditions, and the PHB volume yield is 9.6 times and 1.4 times of that of wild control bacteria W3110/pBHR68(2 percent). |
| priorityDate | 2019-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.