http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110662845-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2ff57144603c661f4b368824eae0729f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_faa6baf301d62173a3e26178954db021 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 |
filingDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b817c668a3b187ac2e89a2229e0d90d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_103b5ba8bc4752f19b1eb823d15e0ab6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_058eb4565c16d0f7c0c1167ebf95dae8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89743437a6539b3c4d4d2707c8318317 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9c067bc16094fc9a1db75b3fa3e7ff0 |
publicationDate | 2020-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110662845-A |
titleOfInvention | Characterization of DNA methylation using magnetoresistive biosensor arrays |
abstract | A method for methylation detection provides a quantitative description of methylation density in DNA strands. Bisulfite conversion [100] of DNA strands containing methylated and unmethylated sites yields transformed DNA strands with mismatched base pairs. Transformed DNA strands are PCR amplified [102] and magnetically labeled [104] and hybridized [106] with complementary DNA immobilized on a magnetoresistive (MR) sensor array to single-stranded target DNA strands. During hybridization, the binding signal can be recorded. Stringent conditions such as temperature or salt concentration are increased [108] to denature magnetically labeled single-stranded target DNA strands from complementary DNA strands immobilized on a magnetoresistive (MR) sensor array. During the increase in stringency conditions, the denaturation signal generated from the denatured magnetically labeled single-stranded target DNA strands is recorded in real time [110] and used to determine stringency conditions for methylated and unmethylated DNA strands [112]. DNA strands may also contain wild-type genes and mutated genes, so mutation sites can be identified at the same time as methylation sites. |
priorityDate | 2017-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 307.