http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110643702-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e5ffa95ac0db13bc0ea54b430f162d3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
| filingDate | 2018-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f0c4ba97d5f9058f6453ac790d6de4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_167688700a90df49fea57a4d81f50313 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bdbe623bab172c6d6ec0042f98eaf91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19d27edb3a7d64b216ee81d68470e3b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd28244f483f687c7015db814f15ed22 |
| publicationDate | 2020-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110643702-A |
| titleOfInvention | Method for determining methylation level of DNA of specific site in biological sample and application thereof |
| abstract | The invention discloses a method for measuring the methylation level of DNA of a specific site in a biological sample and application thereof. The method comprises the following steps: obtaining a biological sample, and extracting a DNA fragment to be detected in the biological sample; modifying a DNA segment to be detected, and converting unmethylated cytosine deamination in the DNA segment to be detected into uracil; taking the modified DNA fragment as a template DNA fragment, and respectively carrying out PCR amplification on the template DNA fragment from two sides of the template DNA fragment by using a first primer and a second primer; processing the PCR product after PCR amplification, removing redundant first primers, second primers and dNTPs, and purifying and recovering; detecting methylation and demethylation fluorescence polarization values of CpG sites of the DNA fragment to be detected; and calculating the methylation level of the DNA fragment to be detected according to the methylation and demethylation fluorescence polarization values of the CpG sites of the DNA fragment to be detected. The method has the advantages of simplicity, convenience, rapidness, high flux, low cost, no need of separation and purification and the like, and is suitable for detecting methylation of a large amount of clinically precious low-concentration DNA samples. |
| priorityDate | 2018-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.