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filingDate 2019-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b99c8e65fdb41185c8a8b09ebada7ce
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publicationDate 2020-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110643674-A
titleOfInvention A rapid method for determining the total number of bacteria on the surface of biological activated carbon filter
abstract The invention relates to a method for rapidly measuring the total number of bacteria on the surface of a biological activated carbon filter. (2) Centrifuge the bacterial desorption solution obtained in step (1) at 4°C, centrifuge at 6000 r/min for 10 min to obtain precipitated bacterial cells, reconstitute the precipitated bacterial cells with lysate, and vortex Mix well, after 30-40 min in a water bath at 60-80 °C, cool to room temperature, centrifuge at 10,000 r/min for 10 min at 4 °C, transfer the supernatant to a 96-well plate, and immediately use the multi-function plate reader fluorescence mode to measure NADH, The excitation wavelength is 350-400 nm, and the emission wavelength is 400-650 nm. Compared with the HPC method and the ATP method, the bacterial value obtained by the detection method of the present invention has a good linear relationship, and can be suitable for the rapid detection of the total bacterial amount in the biological activated carbon filter.
priorityDate 2019-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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