http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110643674-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0ee33b0e3e7398cf6fc957eeee59d510 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate | 2019-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b99c8e65fdb41185c8a8b09ebada7ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57c1bf142bc3a93b881caa08f6476a94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a8b2a9af807711efd00973293a6739c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4664a22f83015f7ef2dfe87644a71b85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_895a0d4f4fb705556d62cc97b9e2b186 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e89653e16ef260e28f5332f3e070fa67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cecadab1dcc9d774f0f736c59742595 |
publicationDate | 2020-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110643674-A |
titleOfInvention | A rapid method for determining the total number of bacteria on the surface of biological activated carbon filter |
abstract | The invention relates to a method for rapidly measuring the total number of bacteria on the surface of a biological activated carbon filter. (2) Centrifuge the bacterial desorption solution obtained in step (1) at 4°C, centrifuge at 6000 r/min for 10 min to obtain precipitated bacterial cells, reconstitute the precipitated bacterial cells with lysate, and vortex Mix well, after 30-40 min in a water bath at 60-80 °C, cool to room temperature, centrifuge at 10,000 r/min for 10 min at 4 °C, transfer the supernatant to a 96-well plate, and immediately use the multi-function plate reader fluorescence mode to measure NADH, The excitation wavelength is 350-400 nm, and the emission wavelength is 400-650 nm. Compared with the HPC method and the ATP method, the bacterial value obtained by the detection method of the present invention has a good linear relationship, and can be suitable for the rapid detection of the total bacterial amount in the biological activated carbon filter. |
priorityDate | 2019-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.