http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110643566-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8214 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 |
| filingDate | 2019-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110643566-B |
| titleOfInvention | A kind of rice protoplast isolation and transformation method |
| abstract | The invention discloses a rice protoplast separation and transformation method. It relates to the technical field of protoplast separation and transformation. The separation method is to take the stems and leaves of rice seedlings and prepare them into flakes, enzymolysis, sieving, discarding the filtrate, and leaving the remaining flakes; elution and recovery of the remaining flakes after enzymolysis with W5 solution, shaking, sieving to collect the filtrate, All the collected filtrates were mixed, centrifuged, and the supernatant was discarded to obtain a precipitate, which was resuspended with the W5 solution, centrifuged to discard the supernatant, and mixed with the MMg solution. The transformation method is to mix the target vector and the protoplast suspension, mix with PEG, water bath, and mix with W5 solution, and stop the reaction, centrifuge, discard the supernatant, mix with W5 solution, and transfer to cell culture plate, Cultivated in the dark. The invention can better obtain high-quality rice protoplasts and apply them to subsequent research. |
| priorityDate | 2019-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.