http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110643566-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a483f83e8ee0d67e3e5da851a1455037 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8214 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2019-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73cb288af17324d50b8bb6b544df625c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba488ce912260f6519112808020ca305 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35e45f141232a48262e2eaa3aabbfc00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ae36dc4b1b88066b4d7acfd6551c5fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22c0fde877a2d90073600f7e67768a22 |
| publicationDate | 2020-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110643566-A |
| titleOfInvention | A kind of rice protoplast isolation and transformation method |
| abstract | The invention discloses a rice protoplast separation and transformation method. It relates to the technical field of protoplast separation and transformation. The separation method is to take the stems and leaves of rice seedlings and prepare them into flakes, enzymolysis, sieving, discarding the filtrate, and leaving the remaining flakes; the flakes remaining after enzymatic hydrolysis are eluted and recovered with W5 solution, shaken, sieved to collect the filtrate, and All the collected filtrates were mixed, centrifuged, and the supernatant was discarded to obtain a precipitate, which was resuspended with the W5 solution, centrifuged to discard the supernatant, and mixed with the MMg solution. The transformation method is to mix the target vector and the protoplast suspension, mix with PEG, water bath, and mix with W5 solution, and stop the reaction, centrifuge, discard the supernatant, mix with W5 solution, and transfer it into a cell culture plate, Cultivated in the dark. The invention can better obtain high-quality rice protoplasts and apply them to subsequent research. |
| priorityDate | 2019-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.