http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110632041-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6f1c3a7dee856261628f5d952300ad5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2019-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_936dd08cce7d15ac609a00e942c7bd13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b03252b354fb7c833f1bd80be737efd9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ce940b3774125cb5fcb48dc520c8f53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_149f83643813f5a70fdac49924b41710 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9761e9f27fc7dd6bf939428687fcd0e2 |
| publicationDate | 2019-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110632041-A |
| titleOfInvention | A kind of determination method of lycorine as fluorescent probe for detecting hydroxyl radical |
| abstract | The invention discloses a method for measuring lycorine as a fluorescent probe for detecting hydroxyl radicals, through the fluorescence response of lycorine to hydroxyl radicals, and the relationship between the fluorescence intensity of lycorine and the amount of added hydroxyl radicals, Capable of accurately quantifying hydroxyl radicals at the micromolar level. It specifically includes the following steps: first determine the excitation and emission wavelengths of lycorine, and then perform fluorescence response detection on lycorine with different ions, different concentrations of hydrogen peroxide and different concentrations of hydroxyl radicals, and obtain 1.176 μM lycorine. Hydrogen peroxide responds to hydroxyl radicals produced by ferrous sulfate, and can be used as a fluorescent probe for hydroxyl radicals. It has no fluorescent response to hydrogen peroxide, and cannot be used as a fluorescent probe for hydrogen peroxide and the fluorescence of lycorine. There is a quadratic functional relationship between the intensity and the concentration of added hydroxyl radicals, which shows that lycorine can be used as a fluorescent probe for hydroxyl radicals, and the detection is very sensitive, and it can accurately quantify micromolar hydroxyl radicals. |
| priorityDate | 2019-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.