http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110628804-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
filingDate | 2019-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110628804-B |
titleOfInvention | Method for constructing saccharomyces cerevisiae strain with high tolerance to isobutanol |
abstract | The invention discloses a method for constructing a saccharomyces cerevisiae strain with high tolerance to isobutanol, which relates to a technology for introducing mutant DNA (deoxyribonucleic acid) recombination into a yeast by using a carrier, transforms a saccharomyces cerevisiae cell by using molecular biology and gene recombination technology, converts a plasmid YCplac22-spt15-30 of an over-expression saccharomyces cerevisiae mutant gene spt15-30 into a host bacterium, namely a saccharomyces cerevisiae wild strain W303-1A, and screens a transformant with the plasmid after culturing for 2-3 days on a CM-Tryptophan solid culture medium to construct the saccharomyces cerevisiae strain HBGDAL-104 with high tolerance to isobutanol. The invention overcomes the defects that the yield of the isobutanol is reduced in the later fermentation period and the tolerance of the existing saccharomyces cerevisiae strain for producing the isobutanol cannot adapt to the requirement of further improving the yield of the isobutanol in the later fermentation period in the prior art. |
priorityDate | 2019-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.