| abstract |
The invention belongs to the technical field of early sex identification of birds, and in particular relates to a PCR method for nondestructively identifying the early sex of monosexual birds. The method collects feathers containing feather pith of early birds, extracts DNA by a simple and efficient method, uses the extracted DNA as a template for PCR amplification with PCR amplification primers, and then conducts agarose gel electrophoresis on the amplified products If the amplification result is a single band, it is judged as male, and the double band is judged as female. Compared with the existing method, the method of the present invention can extract significantly higher DNA concentration and purity, and the effect is obviously better than other methods. High-volume gender identification. |